The sensitivity of multiple ecotoxicological assays for evaluating Microcystis aeruginosa cellular algal organic matter and contribution of cyanotoxins to the toxicity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542833" target="_blank" >RIV/61388971:_____/21:00542833 - isvavai.cz</a>

Alternative codes found

RIV/67985874:_____/21:00542833 RIV/00216208:11310/21:10430478

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0041010121000763" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0041010121000763</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxicon.2021.03.003" target="_blank" >10.1016/j.toxicon.2021.03.003</a>

Result language

angličtina

Original language name

The sensitivity of multiple ecotoxicological assays for evaluating Microcystis aeruginosa cellular algal organic matter and contribution of cyanotoxins to the toxicity

Original language description

Secondary metabolites of cyanobacteria and algae released during algal blooms often exhibit toxic effects, but only a small number of the metabolites are the subject of routine analytical screenings. Alternatively, ecotoxicological assays offer a better representation of the overall negative effects. The aim of this work was to compare multiple assays in their sensitivity towards cellular algal organic matter (COM) of the toxin-producing cyanobacterium Microcystis aeruginosa. Multiple endpoints were investigated: mortality, growth inhibition, bioluminescence inhibition, genotoxicity, endocrine-disrupting effects, oxidative stress, and the induction of ethoxyresorufin-O-deethylase (EROD). Three rainbow trout (Oncorhynchus mykiss) cell lines as well as representatives of bacteria, yeasts, algae, vascular plants, and crustaceans were employed, and the results were expressed per mg of dissolved organic carbon (DOC) in the COM. M. aeruginosa COM was toxic to the RTgill-W1, RTG-2, and RTL-W1 cell lines (EC50 values ranging from 0.48 +/- 0.02 to 1.9 +/- 0.1 mg(DOC)/L), to the crustacean Thamnocephalus platyurus (LC50 = 20 +/- 1 mg(DOC)/L), and to Lepidium sativum (IC50 = 241 +/- 13 mg(DOC)/L). In contrast, no effect was observed for bacteria and yeasts, and the growth of the alga Desmodesmus subspicatus was even stimulated. No genotoxicity, endocrine-disrupting effects or increase in oxidative stress or EROD activity was detected. The content of six microcystins (MC-LR, MC-RR, MC-YR, MC-LY, MC-LW, and MC-LF), anatoxin-a, cylindrospermopsin, and nodularin in the M. aeruginosa COM was determined by liquid chromatography-tandem mass spectrometry. An artificially prepared mixture of the detected cyanotoxins in the corresponding concentrations did not induce response in the O. mykiss cell lines and T. platyurus, suggesting that other cyanobacterial metabolites are responsible for the toxicity of M. aeruginosa.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Project

<a href="/en/project/GA18-14445S" target="_blank" >GA18-14445S: Algal organic matter oxidation and its impact on ecotoxicity and water treatment by coagulation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicon

ISSN

0041-0101

e-ISSN

1879-3150

Volume of the periodical

195

Issue of the periodical within the volume

MAY 2021

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

69-77

UT code for WoS article

000637220900010

EID of the result in the Scopus database

2-s2.0-85102801768