Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00542929" target="_blank" >RIV/61388971:_____/21:00542929 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/21:00542929 RIV/68378041:_____/21:00542929 RIV/00064190:_____/21:N0000060 RIV/75010330:_____/21:00013475 and 3 more

Result on the web

<a href="https://www.mdpi.com/2072-6694/13/10/2463" target="_blank" >https://www.mdpi.com/2072-6694/13/10/2463</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cancers13102463" target="_blank" >10.3390/cancers13102463</a>

Result language

angličtina

Original language name

Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

Original language description

Chronic colitis and colon cancer develop for alteration of the mucosa homeostatic regulation, also involving TGF-beta 1. Dextran sulphate sodium (DSS)-induced colitis and azoxymethane (AOM)-induced colorectal carcinogenesis animal models allow for the investigation of the pathological evolution steps. Since chronic inflammation is a common factor, we aimed to explore in rat models the colon mucosa immunological and structural conditions at one month after the end of the inductions, a transition period between acute effects and established lesions. We found, in comparison to healthy controls, downregulation of inflammatory cytokines (except IL-6) and of TGF-beta 1. At the same time, the collagen scaffold was significantly remodelled in both groups. We conclude that the pro-inflammatory cytokines, in front of a downregulated TGF-beta 1, sustained a smouldering inflammation with structural changes preparing the niche of both pathologies (ulcerative colitis with fibrosis, tumour). The collagen scaffold changes pointing to an unnoticed inflammation may be suggested as a possible pre-neoplastic condition marker.nnContinuous activation of the immune system inside a tissue can lead to remodelling of the tissue structure and creation of a specific microenvironment, such as during the tumour development. Chronic inflammation is a central player in stimulating changes that alter the tissue stroma and can lead to fibrotic evolution. In the colon mucosa, regulatory mechanisms, including TGF-beta 1, avoid damaging inflammation in front of the continuous challenge by the intestinal microbiome. Inducing either DSS colitis or AOM colorectal carcinogenesis in AVN-Wistar rats, we evaluated at one month after the end of each treatment whether immunological changes and remodelling of the collagen scaffold were already in development. At this time point, we found in both models a general downregulation of pro-inflammatory cytokines and even of TGF-beta 1, but not of IL-6.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/IAA500200917" target="_blank" >IAA500200917: Genetics and immunity in early stages of colorectal adenocarcinoma: inflammatory environment inconventional vs germ-free animal models, and in human samples</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cancers (Basel)

ISSN

2072-6694

e-ISSN

2072-6694

Volume of the periodical

13

Issue of the periodical within the volume

10

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

2463

UT code for WoS article

000654722200001

EID of the result in the Scopus database

2-s2.0-85105863368