eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00543468" target="_blank" >RIV/61388971:_____/21:00543468 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/21:10429307
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/10/5164" target="_blank" >https://www.mdpi.com/1422-0067/22/10/5164</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22105164" target="_blank" >10.3390/ijms22105164</a>
Alternative languages
Result language
angličtina
Original language name
eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules
Original language description
Stress granules (SGs) are membrane-less assemblies arising upon various stresses in eukaryotic cells. They sequester mRNAs and proteins from stressful conditions and modulate gene expression to enable cells to resume translation and growth after stress relief. SGs containing the translation initiation factor eIF3a/Rpg1 arise in yeast cells upon robust heat shock (HS) at 46 degrees C only. We demonstrate that the destabilization of Rpg1 within the PCI domain in the Rpg1-3 variant leads to SGs assembly already at moderate HS at 42 degrees C. These are bona fide SGs arising upon translation arrest containing mRNAs, which are components of the translation machinery, and associating with P-bodies. HS SGs associate with endoplasmatic reticulum and mitochondria and their contact sites ERMES. Although Rpg1-3-labeled SGs arise at a lower temperature, their disassembly is delayed after HS at 46 degrees C. Remarkably, the delayed disassembly of HS SGs after the robust HS is reversed by TDP-43, which is a human protein connected with amyotrophic lateral sclerosis. TDP-43 colocalizes with HS SGs in yeast cells and facilitates cell regrowth after the stress relief. Based on our results, we propose yeast HS SGs labeled by Rpg1 and its variants as a novel model system to study functions of TDP-43 in stress granules disassembly.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA16-05497S" target="_blank" >GA16-05497S: Stress granule dynamics and heat stress survival</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
22
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
27
Pages from-to
5164
UT code for WoS article
000662007800001
EID of the result in the Scopus database
2-s2.0-85105625226