Laccase and horseradish peroxidase for green treatment of phenolic micropollutants in real drinking water and wastewater

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00544749" target="_blank" >RIV/61388971:_____/21:00544749 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24220/21:00008428 RIV/46747885:24620/21:00008428 RIV/00216208:11310/21:10430084

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11356-021-12910-0" target="_blank" >https://link.springer.com/article/10.1007%2Fs11356-021-12910-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11356-021-12910-0" target="_blank" >10.1007/s11356-021-12910-0</a>

Result language

angličtina

Original language name

Laccase and horseradish peroxidase for green treatment of phenolic micropollutants in real drinking water and wastewater

Original language description

Biologically active micropollutants that contain diverse phenolic/aromatic structures are regularly present in wastewater effluents and are even found in drinking water. Advanced green technologies utilizing immobilized laccase and/or peroxidase, which target these micropollutants directly, may provide a reasonable alternative to standard treatments. Nevertheless, the use of these enzymes is associated with several issues that may prevent their application, such as the low activity of laccase at neutral and basic pH or the necessity of hydrogen peroxide addition as a co-substrate for peroxidases. In this study, the activity of laccase from Trametes versicolor and horseradish peroxidase was evaluated across a range of commonly used substrates (2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), syringaldazine, and guaiacol). Moreover, conditions for their optimal performance were explored along with an assessment of whether these conditions accurately reflect the effectivity of both enzymes in the degradation of a mixture of bisphenol A, 17 alpha-ethinylestradiol, triclosan, and diclofenac in tap drinking water and secondary wastewater effluent. Laccase and horseradish peroxidase showed optimal activity at strongly acidic pH if ABTS was used as a substrate. Correspondingly, the activities of both enzymes detected using ABTS in real waters were significantly enhanced by adding approximately 2.5% (v/v) of McIlvaine's buffer. Degradation of a mixture of micropollutants in wastewater with 2.5% McIlvaine's buffer (pH 7) resulted in a substantial decrease in estrogenic activity. Low degradation efficiency of micropollutants by laccase was observed in pure McIlvaine's buffer of pH 3 and 7, compared with efficient degradation in tap water of pH 7.5 without buffer.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20801 - Environmental biotechnology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Science and Pollution Research

ISSN

0944-1344

e-ISSN

1614-7499

Volume of the periodical

28

Issue of the periodical within the volume

24

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

31566-31574

UT code for WoS article

000619737500021

EID of the result in the Scopus database

2-s2.0-85101199373