Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00545726" target="_blank" >RIV/61388971:_____/21:00545726 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10429817
Result on the web
<a href="https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-021-04858-4" target="_blank" >https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-021-04858-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13071-021-04858-4" target="_blank" >10.1186/s13071-021-04858-4</a>
Alternative languages
Result language
angličtina
Original language name
Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences
Original language description
Background: Phlebotomine sand flies (Diptera: Psychodiae) in the Republic of Moldova have been understudied for decades. Our study provides a first update on their occurrence, species composition and bloodmeal sources after 50 years.nMethods: During 5 seasons (2013-2017), 58 localities from 20 regions were surveyed for presence of sand flies using CDC light traps and manual aspirators. Species identification was done by a combination of morphological and molecular approaches (DNA barcoding, MALDI-TOF MS protein profiling). In engorged females, host blood was identified by three molecular techniques (RFLP, cytb sequencing and MALDI-TOF peptide mass mapping). Population structure of most abundant species was studied by coxl haplotyping, phylogenetic analyses of ITS2 and coxl genetic markers were used to resolve relationships of other detected species.nResults: In total, 793 sand flies were collected at 30 (51.7%) localities from 12 regions of Moldova. Three species were identified by an integrative morphological and molecular approach: Phlebotomus papatasi, P perfiliewi and Phlebotomus sp. (Adlerius), the first being the most abundant and widespread, markedly anthropophilic based on bloodmeal analyses, occurring also indoors and showing low population structure with only five haplotypes of coxl detected. Distinct morphological and molecular characters of Phlebotomus sp. (Adlerius) specimens suggest the presence of a yet undescribed species.nConclusions: Our study revealed the presence of stable sand fly populations of three species in Moldova that represent a biting nuisance as well as a potential threat of pathogen transmission and shall be further studied.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10613 - Zoology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasites & Vectors
ISSN
1756-3305
e-ISSN
1756-3305
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
371
UT code for WoS article
000677616800001
EID of the result in the Scopus database
2-s2.0-85110971627