Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00546810" target="_blank" >RIV/61388971:_____/21:00546810 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-021-92657-3" target="_blank" >https://www.nature.com/articles/s41598-021-92657-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-021-92657-3" target="_blank" >10.1038/s41598-021-92657-3</a>
Alternative languages
Result language
angličtina
Original language name
Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment
Original language description
Algae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO2 availability (from 390 to 5000 ppm and vice versa) over 75 min. We used a previously developed algorithm to correct sinusoidal interference fringes from IR hyperspectral imaging data. After preprocessing and fringe correction of the hyperspectral data, principal component analysis (PCA) was performed to assess the spatial distribution of organic pools within the algal cells. Through the analysis of 200,000 spectra, we were able to identify compositional modifications associated with CO2 treatment. PCA revealed changes in the carbohydrate pool (1200-950 cm(-1)), lipids (1740, 2852, 2922 cm(-1)), and nucleic acid (1160 and 1201 cm(-1)) as the major response of exposure to elevated CO2 concentrations. Our results show a local metabolism response to this external perturbation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA17-18917S" target="_blank" >GA17-18917S: Interaction between algae and grazers: sensing the menace and responding to it</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
13246
UT code for WoS article
000670729700023
EID of the result in the Scopus database
2-s2.0-85108678383