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Fecal microbiome changes and specific anti-bacterial response in patients with ibd during anti-tnf therapy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00551610" target="_blank" >RIV/61388971:_____/21:00551610 - isvavai.cz</a>

  • Alternative codes found

    RIV/00023001:_____/21:00081909 RIV/00216208:11110/21:10434244 RIV/00064211:_____/21:W0000004 RIV/00216208:11130/21:10434244

  • Result on the web

    <a href="https://www.mdpi.com/2073-4409/10/11/3188" target="_blank" >https://www.mdpi.com/2073-4409/10/11/3188</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/cells10113188" target="_blank" >10.3390/cells10113188</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Fecal microbiome changes and specific anti-bacterial response in patients with ibd during anti-tnf therapy

  • Original language description

    Inflammatory bowel diseases (IBD) are chronic disorders of the gastrointestinal tract that have been linked to microbiome dysbiosis and immune system dysregulation. We investigated the longitudinal effect of anti-TNF therapy on gut microbiota composition and specific immune response to commensals in IBD patients. The study included 52 patients tracked over 38 weeks of therapy and 37 healthy controls (HC). To characterize the diversity and composition of the gut microbiota, we used amplicon sequencing of the V3V4 region of 16S rRNA for the bacterial community and of the ITS1 region for the fungal community. We measured total antibody levels as well as specific antibodies against assorted gut commensals by ELISA. We found diversity differences between HC, Crohn’s disease, and ulcerative colitis patients. The bacterial community of patients with IBD was more similar to HC at the study endpoint, suggesting a beneficial shift in the microbiome in response to treatment. We identified factors such as disease severity, localization, and surgical intervention that significantly contribute to the observed changes in the gut bacteriome. Furthermore, we revealed increased IgM levels against specific gut commensals after anti-TNF treatment. In summary, this study, with its longitudinal design, brings insights into the course of anti-TNF therapy in patients with IBD and correlates the bacterial diversity with disease severity in patients with ulcerative colitis (UC).

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/NV18-09-00493" target="_blank" >NV18-09-00493: Microbiota as a biomarker for prediction of mucocutaneous adverse effects of biologics used in inflammatory bowel disease</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Cells

  • ISSN

    2073-4409

  • e-ISSN

    2073-4409

  • Volume of the periodical

    10

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    21

  • Pages from-to

    3188

  • UT code for WoS article

    000749993600001

  • EID of the result in the Scopus database

    2-s2.0-85118973464