Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00553501" target="_blank" >RIV/61388971:_____/22:00553501 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10454373
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1570963921001412?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1570963921001412?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbapap.2021.140735" target="_blank" >10.1016/j.bbapap.2021.140735</a>

Result language
angličtina
Original language name
Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex
Original language description
Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single residue resolution. In the present study, FPOP analysis was applied to study the hemoglobin (Hb) haptoglobin (Hp) complex allowing identification of respective regions altered upon the complex formation. FPOP footprinting using a timsTOF Pro mass spectrometer revealed structural information for 84 and 76 residues in Hp and Hb, respectively, including statistically significant differences in the modification extent below 0.3%. The most affected residues upon complex formation were Met76 and Tyr140 in Hba, and Tyr280 and Trp284 in Hp beta. The data allowed determination of amino acids directly involved in Hb Hp interactions and those located outside of the interaction interface yet affected by the complex formation. Also, previously modeled interaction between Hb beta Trp37 and Hp beta Phe292 was not confirmed by our data. Data are available via ProteomeXchange with identifier PXD021621.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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