Comparing pulse amplitude modulated (PAM) fluorometry with radiocarbon technique for determination of inorganic carbon fixation in Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00554361" target="_blank" >RIV/61388971:_____/22:00554361 - isvavai.cz</a>
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/09670262.2021.1885065?scroll=top&needAccess=true" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/09670262.2021.1885065?scroll=top&needAccess=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09670262.2021.1885065" target="_blank" >10.1080/09670262.2021.1885065</a>
Alternative languages
Result language
angličtina
Original language name
Comparing pulse amplitude modulated (PAM) fluorometry with radiocarbon technique for determination of inorganic carbon fixation in Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)
Original language description
Integration of pulse amplitude modulated (PAM) fluorometry and conventional methods for estimating carbon assimilation in microalgae is important for physiological, ecological and economic purposes. In this study, we compared PAM fluorometry and carbon-14 (C-14) uptake techniques to estimate the carbon fixation rate in Chlorella vulgaris under controlled laboratory conditions. The key parameter for this comparison was the electron yield for carbon fixation (CYRILLIC CAPITAL LETTER EFe ), commonly assumed when converting electron transport rate (ETR) values into the chlorophyll-specific carbon fixation rate (P-B ). Additional analyses of maximum (phi(M) ) and effective (phi'(M) ) quantum efficiency of photosystem II, photochemical (qP) and non-photochemical (NPQ) quenching, and photosynthesis-irradiance response curves demonstrated that the photophysiology of C. vulgaris did not change after a 2-h incubation with (NaHCO3)-C-14 and Na2CO3 (control). The association of P-B obtained through the C-14 method (151 +/- 8.77 mu mol C [mg chl a](-1) h(-1)) with ETR (411 +/- 3.91 mu mol e(-) [mg chl a](-1) h(-1)) resulted in an average CYRILLIC CAPITAL LETTER EFe of 0.37 +/- 0.02 mu mol C [mu mol e(-)](-1), which is higher than theoretical CYRILLIC CAPITAL LETTER EFe values usually reported in the literature (e.g. 0.20 and 0.25). We attributed this discrepancy to a possible inaccuracy in ETR due to underestimated values of chlorophyll-specific absorption cross-section (a*) and the common assumption that only 50% of total light is absorbed by photosystem II. We here demonstrate the importance of associating chlorophyll fluorescence with other primary production techniques, so that adjustments to calculation procedures can be made in accordance to species-specific physiological traits and particularities regarding culturing conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA18-07822S" target="_blank" >GA18-07822S: Strategies of adaptation of eukaryotic phytoplankton to iron limitation</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Phycology
ISSN
0967-0262
e-ISSN
1469-4433
Volume of the periodical
57
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
18-28
UT code for WoS article
000637274600001
EID of the result in the Scopus database
2-s2.0-85103878701