Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00557609" target="_blank" >RIV/61388971:_____/22:00557609 - isvavai.cz</a>
Result on the web
<a href="https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787" target="_blank" >https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/mgen.0.000787" target="_blank" >10.1099/mgen.0.000787</a>
Alternative languages
Result language
angličtina
Original language name
Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales
Original language description
The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae. Here, we show that two large natural RepABCtype plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co- cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co- culture.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbial Genomics
ISSN
2057-5858
e-ISSN
2057-5858
Volume of the periodical
8
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
000787
UT code for WoS article
000788985200003
EID of the result in the Scopus database
2-s2.0-85125871952