Periphyton (microbiome) of filamentous algae studied by polarization microscopy, aided also by some contrasting optical methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00571668" target="_blank" >RIV/61388971:_____/23:00571668 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s12223-022-01011-1" target="_blank" >https://link.springer.com/article/10.1007/s12223-022-01011-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-022-01011-1" target="_blank" >10.1007/s12223-022-01011-1</a>

Result language

angličtina

Original language name

Periphyton (microbiome) of filamentous algae studied by polarization microscopy, aided also by some contrasting optical methods

Original language description

Polarization microscopy, possibly together with some contrast techniques (dark field and color phase contrast), was used to study the periphyton (microbiome) growing on filamentous green algae. The material containing filamentous algae with periphyton on the surface was collected in the villages of SATIN SMALL LETTER Y WITH ACUTEkorice and Zbecno (Krivoklatsko Protected Landscape Area). The objects were studied in a LOMO MIN-8 St. Petersburg polarizing microscope and a Carl Zeiss Jena NfpK laboratory microscope equipped with an In Ph 160 basic body with variable dark field or color phase contrast and a Nikon D70 DSLR digital camera. Cells of filamentous algae of the genera Cladophora, Vaucheria, and Oedogonium were studied and the periphyton attached to them formed by cyanobacteria of the genera Chamaesiphon and Pleurocapsa and algae of the genera Characium, including diatoms of the genera Eunotia and Synedra. In all cases, the cell walls of the host algae showed a very strong birefringence. In contrast, the walls of cyanobacteria of the genera Chamaesiphon and Pleurocapsa were characterized by a much weaker birefringence (Pleurocapsa somewhat thicker), and the diatom frustules of the genera Eunotia and Synedra were almost without a birefringence. Strongly birefringent granules were found in the cytoplasm of the green alga of the genus Characium, which forms periphyton on the filamentous green algae of the genus Vaucheria. The periphyton on the filamentous alga of the genus Oedogonium, formed by cyanobacteria of the genus Pleurocapsa and diatoms of the genera Eunotia and Synedra, deposited in a massive layer of mucus containing birefringent crystals, showed a particularly strong birefringence. At the end of the vegetation of filamentous algae, their parts and remnants of periphyton (diatom frustules and crystals) became part of the detritus at the bottom of the culture vessel. The use of polarization microscopy in the study of filamentous algae with periphyton on the surface allows us not only to determine the birefringence of the observed structures, but also to partially deduce their chemical composition, or regular arrangement of particles, so-called shape birefringence.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA21-17044S" target="_blank" >GA21-17044S: Next-generation infection diagnostics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

1874-9356

Volume of the periodical

68

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

291-298

UT code for WoS article

000871851200001

EID of the result in the Scopus database

2-s2.0-85140610438