Detection of galanin receptors in the spinal cord in experimental autoimmune encephalomyelitis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00575464" target="_blank" >RIV/61388971:_____/23:00575464 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/23:10442537 RIV/00064165:_____/23:10442537

Result on the web

<a href="https://biomed.papers.upol.cz/artkey/bio-202301-0006_detection-of-galanin-receptors-in-the-spinal-cord-in-experimental-autoimmune-encephalomyelitis.php" target="_blank" >https://biomed.papers.upol.cz/artkey/bio-202301-0006_detection-of-galanin-receptors-in-the-spinal-cord-in-experimental-autoimmune-encephalomyelitis.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2022.005" target="_blank" >10.5507/bp.2022.005</a>

Result language

angličtina

Original language name

Detection of galanin receptors in the spinal cord in experimental autoimmune encephalomyelitis

Original language description

Aims. The neuropeptide galanin is a widely distributed neurotransmitter/neuromodulator that regulates a variety of physiological processes and also participates in the regulation of stress responses. The aims of the present study were to investigate the expression of galanin receptors (GalR1, GalR2, GalR3) in the spinal cords in a murine model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE) using qPCR analysis and to determine GalR1 cellular localization (oligodendrocytes, microglia, astrocytes, ependymal cells, and endothelial cells in the capillaries) by immunohistochemistry. Methods. Twelve samples from the EAE group and 14 samples from the control group were analyzed. Spinal cords samples were obtained at the peak of the EAE disease. Results. The GalR1 mRNA level was significantly decreased in the EAE mice compared with the controls (P=0.016), whereas the mRNA levels of GalR2 and GalR3 were not significantly different for the EAE and the control mice. No significant correlations were found between the severity of the EAE disease and the mRNA levels of GalR1, GalR2 and GalR3. Immunochemical detection of the GalR1 revealed its expression in the ependymal and endothelial cells. Additionally, a weak GalR1 immunoreactivity was occasionally detected in the oligodendrocytes. Conclusion. This study provides additional evidence of galanin involvement in EAE pathophysiology, but this has to be further investigated.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers

ISSN

1213-8118

e-ISSN

1804-7521

Volume of the periodical

167

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

36-42

UT code for WoS article

001055136000006

EID of the result in the Scopus database

2-s2.0-85145003630