Terbinafine resistance in Trichophyton mentagrophytes and Trichophyton rubrum in the Czech Republic: A prospective multicentric study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00584095" target="_blank" >RIV/61388971:_____/24:00584095 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17110/24:A2503AEO RIV/00216208:11110/24:10479459 RIV/00216208:11310/24:10479459 RIV/00064165:_____/24:10479459 RIV/71009396:_____/24:N0000016
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/myc.13708" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/myc.13708</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/myc.13708" target="_blank" >10.1111/myc.13708</a>
Alternative languages
Result language
angličtina
Original language name
Terbinafine resistance in Trichophyton mentagrophytes and Trichophyton rubrum in the Czech Republic: A prospective multicentric study
Original language description
Background: Terbinafine, an allylamine antifungal, is crucial for treating dermatophytosis by inhibiting squalene epoxidase (SQLE) in the ergosterol biosynthetic pathway. However, resistance is emerging, particularly in India and Southeast Asia, but reports of resistance spread worldwide. Despite this, comprehensive studies on terbinafine resistance in Trichophyton are still limited. Objectives: This research aimed to determine the prevalence of terbinafine resistance in the Czech Republic, with a focus on Trichophyton rubrum and Trichophyton mentagrophytes, and investigate the underlying molecular mechanisms. Patients/Methods: A total of 514 clinical strains of T. rubrum and 240 T. mentagrophytes collected from four Czech clinical institutions were screened for terbinafine resistance. Molecular investigations included DNA sequencing, specifically the ITS rDNA region and SQLE gene, as well as antifungal susceptibility testing following EUCAST guidelines. Results: While no resistance was observed in T. rubrum, 2.5% of T. mentagrophytes strains exhibited resistance, marked by the F397L mutation in SQLE. Notably, resistance surged from 1.2% in 2019 to 9.3% in 2020 but reverted to 0% in 2021. All resistant strains were identified as T. mentagrophytes var. indotineae. Resistant strains exhibited high MICs for terbinafine (>= 4 mg L-1) but low MICs to the other seven antifungals tested except for fluconazole. Conclusions: This study highlights the emergence of terbinafine-resistant T. mentagrophytes strains in the Czech Republic, with the F397L mutation being pivotal. Due to the relatively low resistance level, the current guidelines for dermatomycosis treatment in the Czech Republic remain effective, but ongoing surveillance is essential for timely adaptations if resistance patterns change.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NU21-05-00681" target="_blank" >NU21-05-00681: Consequences of hybridization for spread of antifungal resistance and species delimitation in pathogenic fungi: model study in Aspergillus and Trichophyton</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mycoses
ISSN
0933-7407
e-ISSN
1439-0507
Volume of the periodical
67
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
e13708
UT code for WoS article
001177489500002
EID of the result in the Scopus database
2-s2.0-85186208051