Design of AsLOV2 domain as a carrier of light-induced dissociable FMN photosensitizer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00584828" target="_blank" >RIV/61388971:_____/24:00584828 - isvavai.cz</a>
Alternative codes found
RIV/00216224:90127/24:00139163 RIV/00216208:11310/24:10478173
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/pro.4921" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/pro.4921</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/pro.4921" target="_blank" >10.1002/pro.4921</a>
Alternative languages
Result language
angličtina
Original language name
Design of AsLOV2 domain as a carrier of light-induced dissociable FMN photosensitizer
Original language description
Flavin mononucleotide (FMN) is a highly efficient photosensitizer (PS) yielding singlet oxygen (O-1(2)). However, its O-1(2) production efficiency significantly decreases upon isoalloxazine ring encapsulation into the protein matrix in genetically encoded photosensitizers (GEPS). Reducing isoalloxazine ring interactions with surrounding amino acids by protein engineering may increase 1O2 production efficiency GEPS, but at the same time weakened native FMN-protein interactions may cause undesirable FMN dissociation. Here, in contrast, we intentionally induce the FMN release by light-triggered sulfur oxidation of strategically placed cysteines (oxidation-prone amino acids) in the isoalloxazine-binding site due to significantly increased volume of the cysteinyl side residue(s). As a proof of concept, in three variants of the LOV2 domain of Avena sativa (AsLOV2), namely V416C, T418C, and V416C/T418C, the effective O-1(2) production strongly correlated with the efficiency of irradiation-induced FMN dissociation (wild type (WT) < V416C < T418C < V416C/T418C). This alternative approach enables us: (i) to overcome the low O-1(2) production efficiency of flavin-based GEPSs without affecting native isoalloxazine ring-protein interactions and (ii) to utilize AsLOV2, due to its inherent binding propensity to FMN, as a PS vehicle, which is released at a target by light irradiation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LX22NPO5107" target="_blank" >LX22NPO5107: National institute for Neurological Research</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Science
ISSN
0961-8368
e-ISSN
1469-896X
Volume of the periodical
33
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
e4921
UT code for WoS article
001187153900001
EID of the result in the Scopus database
2-s2.0-85188256683