Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00585159" target="_blank" >RIV/61388971:_____/24:00585159 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/24:00585159 RIV/00216208:11310/24:10495579

Result on the web

<a href="https://doi.org/10.1002/adfm.202314088" target="_blank" >https://doi.org/10.1002/adfm.202314088</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adfm.202314088" target="_blank" >10.1002/adfm.202314088</a>

Result language

angličtina

Original language name

Design Rules for the Nano-Bio Interface of Nanodiamonds: Implications for siRNA Vectorization

Original language description

The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval of several small interfering RNA (siRNA)-based drugs. However, the efficacy of siRNA delivery is still limited, and an extensive search for alternative and highly effective delivery approaches is ongoing. With this aim, three generations of non-viral vectors based on modified nanodiamonds (NDs) have been gradually developed in the past decade. They show great promise due to the negligible toxicity of the ND core. Here, a robust methodological approach is presented to enable the evaluation of new vector nanomaterials. Using a new type of third-generation ND vector coated with a copolymer with tunable charge density, variables such as colloidal stability, surface electrostatic properties, the molecular composition of the copolymer, and the mode of complexation with siRNA are optimized. Using an innovative data processing strategy, the results are related to biological potency, toxicity, and cell proliferation. Finally, the optimized composition of a coating copolymer consisting of a cationic component, 2-dimethylaminoethyl methacrylate, and an electroneutral biocompatible component, N-(2-hydroxypropyl) methacrylamide, is evaluated. The optimized NDs vectors are colloidally and biologically stable siRNA delivery tools with broad potential for RNA interference-based therapeutics.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advanced Functional Materials

ISSN

1616-301X

e-ISSN

1616-3028

Volume of the periodical

34

Issue of the periodical within the volume

30

Country of publishing house

DE - GERMANY

Number of pages

15

Pages from-to

2314088

UT code for WoS article

001188966200001

EID of the result in the Scopus database

2-s2.0-85188542010