Impaired Proliferation of CD8+T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00588254" target="_blank" >RIV/61388971:_____/24:00588254 - isvavai.cz</a>

Alternative codes found

RIV/00064203:_____/24:10482216 RIV/00216208:11130/24:10482216

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1155/2024/5537948" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/2024/5537948</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2024/5537948" target="_blank" >10.1155/2024/5537948</a>

Result language

angličtina

Original language name

Impaired Proliferation of CD8+T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells

Original language description

CD8(+) T cells are essential for adaptive immunity against infection and tumors. Their ability to proliferate after stimulation is crucial to their functionality. Dendritic cells (DCs) are professional antigen-presenting cells that induce their proliferation. Here, we show that thapsigargin-induced LAD2 mast cell (MC) line-released products can impair the ability of monocyte-derived DCs to induce CD8(+) T-cell proliferation and the generation of Th1 cytokine-producing T cells. We found that culture medium conditioned with LAD2 MCs previously stimulated with thapsigargin (thapsLAD2) induces maturation of DCs as determined by the maturation markers CD80, CD83, CD86, and HLA-DR. However, thapsLAD2-matured DCs produced no detectable TNF alpha or IL-12 during the maturation. In addition, although their surface expression of PD-L1 was comparable with the immature or TLR7/8-agonist (R848)-matured DCs, their TIM-3 expression was significantly higher than in immature DCs and even much higher than in R848-matured DCs. In addition, contrary to R848-matured DCs, the thapsLAD2-matured DCs only tended to induce enhanced proliferation of CD4+ T cells than immature DCs. For CD8(+) T cells, this tendency was not even detected because thapsLAD2-matured and immature DCs comparably induced their proliferation, which contrasted with the significantly enhanced proliferation induced by R848-matured DCs. Furthermore, these differences were comparably recapitulated in the ability of the tested DCs to induce IFN gamma- and IFN gamma/TNF alpha-producing T cells. These findings show a novel mechanism of MC-mediated regulation of adaptive immune responses.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Immunology Research

ISSN

2314-8861

e-ISSN

2314-7156

Volume of the periodical

2024

Issue of the periodical within the volume

July 18

Country of publishing house

GB - UNITED KINGDOM

Number of pages

20

Pages from-to

5537948

UT code for WoS article

001279297200001

EID of the result in the Scopus database

2-s2.0-85199668661