How to best detect threatened deadwood fungi Comparing metabarcoding and fruit body surveys
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00588260" target="_blank" >RIV/61388971:_____/24:00588260 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0006320724002581?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0006320724002581?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biocon.2024.110696" target="_blank" >10.1016/j.biocon.2024.110696</a>
Alternative languages
Result language
angličtina
Original language name
How to best detect threatened deadwood fungi Comparing metabarcoding and fruit body surveys
Original language description
Effective conservation strategies are needed to prevent further loss of biodiversity. This requires a comprehensive assessment of species' presence, distribution, and population sizes. Such assessments can be extremely challenging for species-rich taxa, like fungi, which are difficult to detect and identify. In recent years, metabarcoding applied to environmental samples has proven to be a promising method for fungal detection. However, its potential against traditional fruit body surveys in monitoring threatened fungal species has rarely been tested. Here, we utilized data on deadwood-inhabiting fungi from 569 deadwood objects across five sites in Central Europe to compare the effectiveness of fruit body surveys and metabarcoding (of low and high sampling intensity) in detecting threatened species. Across objects, sites, and per object, metabarcoding was more effective in detecting threatened species than fruit body surveys, regardless of sampling intensity. Eleven percent of all threatened species across all objects and sites could be detected by both methods, 70 % exclusively by metabarcoding and 18 % exclusively by fruit body surveys. The number of species detected by both methods on the same object was <4 %. Effects of high sampling intensity metabarcoding were stronger than low sampling intensity at object level. The effectiveness of the survey method was mainly independent of deadwood object characteristics. We suggest that metabarcoding is a valuable tool for threatened fungal species monitoring and conservation status assessments. However, for a comprehensive assessment, fruit body surveys are still needed, as this method detected a unique set of species and indicates the presence of vital fungal individuals.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biological Conservation
ISSN
0006-3207
e-ISSN
1873-2917
Volume of the periodical
296
Issue of the periodical within the volume
August 2024
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
13
Pages from-to
110696
UT code for WoS article
001270288700001
EID of the result in the Scopus database
2-s2.0-85198086523