New Bacterial Aryl Sulfotransferases: Effective Tools for Sulfation of Polyphenols

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00599437" target="_blank" >RIV/61388971:_____/24:00599437 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10486980 RIV/60461373:22330/24:43930097
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.jafc.4c06771" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jafc.4c06771</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jafc.4c06771" target="_blank" >10.1021/acs.jafc.4c06771</a>

Result language
angličtina
Original language name
New Bacterial Aryl Sulfotransferases: Effective Tools for Sulfation of Polyphenols
Original language description
The preparation of pure metabolites of bioactive compounds, particularly (poly)phenols, is essential for the accurate determination of their pharmacological profiles in vivo. Since the extraction of these metabolites from biological material is tedious and impractical, they can be synthesized enzymatically in vitro by bacterial PAPS-independent aryl sulfotransferases (ASTs). However, only a few ASTs have been studied and used for (poly)phenol sulfation. This study introduces new fully characterized recombinant ASTs selected according to their similarity to the previously characterized ASTs. These enzymes, produced in Escherichia coli, were purified, biochemically characterized, and screened for the sulfation of nine flavonoids and two phenolic acids using p-nitrophenyl sulfate. All tested compounds were proved to be substrates for the new ASTs, with kaempferol and luteolin being the best converted acceptors. ASTs from Desulfofalx alkaliphile (DalAST) and Campylobacter fetus (CfAST) showed the highest efficiency in the sulfation of tested polyphenols. To demonstrate the efficiency of the present sulfation approach, a series of new authentic metabolite standards, regioisomers of kaempferol sulfate, were enzymatically produced, isolated, and structurally characterized.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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