Differential effect of monoterpenes and flavonoids on the transcription of aromatic ring- hydroxylating dioxygenase genes in Rhodococcus opacus C1 and Rhodococcus sp. WAY2

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00618603" target="_blank" >RIV/61388971:_____/25:00618603 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/25:43931426 RIV/60162694:G33__/25:N0000005 RIV/00216208:11310/25:10500182

Result on the web

<a href="https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001359" target="_blank" >https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001359</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/mgen.0.001359" target="_blank" >10.1099/mgen.0.001359</a>

Result language

angličtina

Original language name

Differential effect of monoterpenes and flavonoids on the transcription of aromatic ring- hydroxylating dioxygenase genes in Rhodococcus opacus C1 and Rhodococcus sp. WAY2

Original language description

Aromatic ring- hydroxylating dioxygenases (ARHDs) play a crucial role in the aerobic biodegradation of both natural and anthropogenic aromatic compounds. Although their ability to process contaminants is not entirely understood, it is thought to have evolved from the transformation of structurally similar secondary plant metabolites (SPMs). Hence, to investigate this connection, we tested a variety of SPMs from the monoterpene and flavonoid classes as carbon sources and transcriptional effectors of several phylogenetically distant ARHD genes involved in the degradation of aromatic pollutants. Specifically, we focused on bphA1, nahA1 and phtA1 in Rhodococcus opacus C1, whose genomic analysis is also presented hereinafter, and bphA1a, nahA1-bphA1b and etbA1ab in Rhodococcus sp. WAY2. Whilst induction was only observed with (R)-carvone for bphA1a and nahA1-bphA1b of strain WAY2, and with p-cymene for nahA1 and nahA1-bphA1b of strains C1 and WAY2, respectively, an extensive inhibition by flavonoids was observed for most of the genes in both strains. To the best of our knowledge, our study is the first to report the effect of flavonoids and monoterpenes on the transcription of nahA1, etbA1 and phtA1 genes. In addition, we show that, in contrast to pseudomonads, many flavonoids inhibit the transcription of the ARHD genes in rhodococci. Thus, our work provides a new perspective on flavonoids as the transcriptional effectors of ARHDs, highlighting the significant variability of these enzymes and the divergent responses that they elicit. Moreover, our results contribute to understanding the complex interactions between microorganisms and SPMs and provide insights into the molecular basis of a number of them.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbial Genomics

ISSN

2057-5858

e-ISSN

2057-5858

Volume of the periodical

11

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

001359

UT code for WoS article

001448509800002

EID of the result in the Scopus database

2-s2.0-86000540771