Evidence of in vitro mecβ-mediated β-lactam antibiotic resistance transfer to Staphylococcus aureus from Macrococcus psychrotolerans. sp. nov., a psychrophilic bacterium from food-producing animals and human clinical specimens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00619316" target="_blank" >RIV/61388971:_____/25:00619316 - isvavai.cz</a>

Result on the web

<a href="https://journals.asm.org/doi/10.1128/aem.01652-24" target="_blank" >https://journals.asm.org/doi/10.1128/aem.01652-24</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/aem.01652-24" target="_blank" >10.1128/aem.01652-24</a>

Result language

angličtina

Original language name

Evidence of in vitro mecβ-mediated β-lactam antibiotic resistance transfer to Staphylococcus aureus from Macrococcus psychrotolerans. sp. nov., a psychrophilic bacterium from food-producing animals and human clinical specimens

Original language description

Macrococci are usually found as commensals on the skin and mucosa of animals and have been isolated from mammal-derived fermented foods, however, they can also act as opportunistic pathogens. Here, we used whole-genome sequenc ing, comparative genomics, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize Macrococcus sp. strains isolated from livestock and human-related specimens. Based on the results of polyphasic taxonomy, we propose the species Macrococcus psychrotolerans sp. nov. (type strain NRL/St 95/376(T) = CCM 8659(T )= DSM 111350(T)) belonging to the Macrococcus caseolyticus phylogenetic clade. It grows at 4 degrees C, and the core genome of the isolates contains suspected genes contributing to low-temperature tolerance. Variable genetic elements include prophages, chromosomal islands, a composite staphylococcal cassette chromosome island, and many plasmids that affect the overall genome expansion and adaptation to specific ecological settings of the studied isolates. Large plasmids carrying the methicillin resistance gene mecB were identified in M. psychrotolerans sp. nov. strains and confirmed as self-transmissi ble to Staphylococcus aureus in vitro. In addition to plasmids with circular topology, a 150-kb-long linear plasmid with 14.1-kb-long inverted terminal repeats, harboring many IS elements and putative genes for a type IV secretion system was revealed. The described strains were isolated from human clinical material, food-producing animals, meat, and a wooden cheese board and have the potential to proliferate at refrigerator temperatures. Their presence in the food chain and human infections indicates that attention needs to be paid to this potential novel opportunistic pathogen.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

1098-5336

Volume of the periodical

91

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

24

Pages from-to

e401652-24

UT code for WoS article

001441424800001

EID of the result in the Scopus database

2-s2.0-105003322582