Protists are key players in the utilization of protein nitrogen in the arbuscular mycorrhizal hyphosphere

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00635576" target="_blank" >RIV/61388971:_____/25:00635576 - isvavai.cz</a>

Result on the web

<a href="https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70153" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70153</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.70153" target="_blank" >10.1111/nph.70153</a>

Result language

angličtina

Original language name

Protists are key players in the utilization of protein nitrogen in the arbuscular mycorrhizal hyphosphere

Original language description

While largely depending on other microorganisms for nitrogen (N) mineralization, arbuscular mycorrhizal fungi (AMF) can transfer N from organic sources to their host plants. Here, we compared N acquisition by the AMF hyphae from chitin and protein sources and assessed the effects of microbial interactions in the hyphosphere. We employed in vitro compartmented microcosms, each containing three distinct hyphosphere compartments amended with different N sources (protein, chitin, or ammonium chloride), one of which was enriched with 15N isotope. All hyphosphere compartments were supplied with Paenibacillus bacteria, with or without the protist Polysphondylium pallidum. We measured the effect of these model microbiomes on the efficiency of 15N transfer to roots via the AMF hyphae. We found that the hyphae efficiently took up N from ammonium chloride, competing strongly with bacteria and protists. Mobilization of 15N from chitin and protein was facilitated by bacteria and protists, respectively. Notably, AMF priming significantly affected the abundance of bacteria and protists in hyphosphere compartments and promoted mineralization of protein N by protists. Subsequently, this N was transferred into roots. Our results provide the first unequivocal evidence that roots can acquire N from proteins present in the AMF hyphosphere and that protists may play a crucial role in protein N mineralization.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

New Phytologist

ISSN

0028-646X

e-ISSN

1469-8137

Volume of the periodical

246

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

2753-2764

UT code for WoS article

001471898800001

EID of the result in the Scopus database

2-s2.0-105003712301