Extracellular Vesicle-Mediated Delivery of AntimiR-Conjugated Bio-Gold Nanoparticles for In Vivo Tumor Targeting

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00638787" target="_blank" >RIV/61388971:_____/25:00638787 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/25:00638787 RIV/46747885:24530/25:00014031

Result on the web

<a href="https://www.mdpi.com/1999-4923/17/8/1015" target="_blank" >https://www.mdpi.com/1999-4923/17/8/1015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/pharmaceutics17081015" target="_blank" >10.3390/pharmaceutics17081015</a>

Result language

angličtina

Original language name

Extracellular Vesicle-Mediated Delivery of AntimiR-Conjugated Bio-Gold Nanoparticles for In Vivo Tumor Targeting

Original language description

Background/Objectives: Extracellular vesicles (EVs) are involved in cell-to-cell communication and delivery of signaling molecules and represent an interesting approach in targeted therapy. This project focused on EV-mediated facilitation and cell-specific delivery of effector antimiR molecules carried by biologically produced gold nanoparticles (AuNPs). Methods: First, we loaded EVs derived from cancer cells 4T1 with AuNPs-antimiR. The AuNPs were also decorated with or without transferrin (Tf) molecules. We examined parental cell-specific delivery of the AuNPs-Tf-antimiR within monocultures as well as co-cultures in vitro. Subsequently, we used autologous EVs containing AuNPs-Tf-antimiR to target tumor cells in a xenograft tumor model in vivo. Efficacy of the antimir transfer was assessed by qPCR and apoptosis assessment. Results: In vitro, EVs loaded with AuNPs-antimiR were internalized only by the parental cells and the AuNPs-antimiR transfer was successful and effective only in EVs that were decorated with Tf. We achieved effective delivery of the antimiR molecule into cancer cells in vivo, which was proved by specific silencing of the target oncogenic miRNA as well as induction of cancer cells apoptosis. Conclusions: EVs represent an interesting and potent way for targeted cargo delivery and personalized medicine. On the other hand, there are various safety and efficacy challenges that remain to be addressed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EH22_010%2F0002357" target="_blank" >EH22_010/0002357: MSCA Fellowships CZ ((Institute of Microbiology of the CAS, v. v. i.)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pharmaceutics

ISSN

1999-4923

e-ISSN

1999-4923

Volume of the periodical

17

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

27

Pages from-to

1015

UT code for WoS article

001557512900001

EID of the result in the Scopus database

2-s2.0-105014386228