Spatial structure of yeast biofilms and the role of cell adhesion across different media

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00640234" target="_blank" >RIV/61388971:_____/25:00640234 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/25:10501740

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2590207525000541?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2590207525000541?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioflm.2025.100306" target="_blank" >10.1016/j.bioflm.2025.100306</a>

Result language

angličtina

Original language name

Spatial structure of yeast biofilms and the role of cell adhesion across different media

Original language description

The ability of yeast cells to adhere to solid surfaces or even penetrate semi-solid surfaces and form multicellular biofilms are critical factors in infection. This study examines the relationship between cell adhesion capability and the ability to create spatially organized biofilms in selected Saccharomyces cerevisiae strains, including clinical isolates, and five Candida species (C. albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis). We assessed cell adhesion to polystyrene surface in four media varying in source of carbon and other nutrients. Using microscopy of vertical cell arrangement profiles within yeast populations grown at the solid-liquid interface, we evaluated their internal organization to determine whether the populations exhibit typical biofilm characteristics, such as the spatial organization of distinct cell types. Results indicate that well adherent S. cerevisiae strains form spatial biofilms with typical internal organization, highlighting strain-specific responses to media composition and supporting the use of natural S. cerevisiae strains for biofilm research. Among Candida species, biofilm formation did not consistently align with adhesion efficiency to plastic, while C. albicans and C. krusei formed spatially structured biofilms on media where they adhered well, C. tropicalis and C. glabrata exhibited efficient adhesion without biofilm structuring. Interestingly, C. parapsilosis formed a structured biofilm despite minimal adhesion. These findings emphasize the role of media composition, particularly components of yeast extract and defined medium for mammalian cell growth RPMI, which differentially impacted adhesion and

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EH22_008%2F0004573" target="_blank" >EH22_008/0004573: Breakthrough Laser Technologies for Smart Manufacturing, Space and Bio-Tech Applications</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biofilm

ISSN

2590-2075

e-ISSN

2590-2075

Volume of the periodical

10

Issue of the periodical within the volume

December 2025

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

100306

UT code for WoS article

001578007200001

EID of the result in the Scopus database

2-s2.0-105010869478