Photophysical consequences of spheroidene reconstitution in LH1 of Rsp. rubrum: improved energy transfer and altered photoprotection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00643021" target="_blank" >RIV/61388971:_____/25:00643021 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/pcp/article-abstract/66/11/1750/8219053?redirectedFrom=fulltext&login=true" target="_blank" >https://academic.oup.com/pcp/article-abstract/66/11/1750/8219053?redirectedFrom=fulltext&login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/pcp/pcaf087" target="_blank" >10.1093/pcp/pcaf087</a>
Alternative languages
Result language
angličtina
Original language name
Photophysical consequences of spheroidene reconstitution in LH1 of Rsp. rubrum: improved energy transfer and altered photoprotection
Original language description
Carotenoids are multifunctional pigments that play indispensable roles in photosynthesis, serving both to harvest light and to safeguard the system against photo-induced damage. In purple photosynthetic bacteria, these pigments, alongside bacteriochlorophyll (BChl) a, initiate the primary photochemical process by capturing solar energy within light-harvesting (LH) complexes. The excitation energy absorbed by carotenoids is efficiently transferred to BChl a and subsequently to the reaction center, where charge separation drives energy conversion. Improving the efficiency of excitation energy transfer (EET) from carotenoids to BChl a is a promising strategy for advancing bio-inspired LH systems and artificial photosynthesis. Here, we reconstituted spheroidene, a carotenoid known to achieve similar to 90% EET efficiency in the LH2 complex of Rhodobacter sphaeroides strain 2.4.1, into the carotenoidless LH1 complex of Rhodospirillum (Rsp.) rubrum strain G9+. This modification was anticipated to enhance EET efficiency relative to the native LH1 complex of Rsp. rubrum strain S1. Fluorescence excitation spectroscopy confirmed an improvement in EET. Surprisingly, sub-nanosecond time-resolved absorption spectroscopy revealed the emergence of a long-lived BChl a cation, an unusual state not typically observed in native systems. This phenomenon coincided with shortened triplet lifetimes of both carotenoid and BChl a, implying altered photoprotective dynamics. These findings suggest that while spheroidene facilitates efficient energy transfer in LH1 from Rsp. rubrum, it may also perturb the native protein environment, potentially compromising photoprotection. Our study underscores the delicate balance between energy transfer and photostability, offering new insights into the design of robust and efficient artificial photosynthetic systems.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant and Cell Physiology
ISSN
0032-0781
e-ISSN
1471-9053
Volume of the periodical
66
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1750-1759
UT code for WoS article
001558196500001
EID of the result in the Scopus database
2-s2.0-105023298936