Protocol to investigate gene expression heterogeneity in cyanobacteria using mRNA CARD-FISH

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00643708" target="_blank" >RIV/61388971:_____/25:00643708 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/25:43910244
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2666166725006185?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2666166725006185?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.xpro.2025.104212" target="_blank" >10.1016/j.xpro.2025.104212</a>

Result language
angličtina
Original language name
Protocol to investigate gene expression heterogeneity in cyanobacteria using mRNA CARD-FISH
Original language description
Here, we present a protocol for visualizing gene expression in the filamentous cyanobacterium Trichodesmium and the single-celled species Synechocystis and Cyanothece using the catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) technique. We describe steps for fixation, agarose coating, enzymatic permeabilization, and sample handling. This protocol is broadly applicable to cyanobacteria, and the detection of rbcL mRNA in Trichodesmium, used as an example, supports its use to study the heterogeneity of physiological processes at single-cell level.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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