Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388998%3A_____%2F21%3A00542937" target="_blank" >RIV/61388998:_____/21:00542937 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/21:00120203

Result on the web

<a href="https://journals.biologists.com/jcs/article-abstract/doi/10.1242/jcs.258228/268958/Divergent-estimates-of-ratio-between-Na-Ca2?redirectedFrom=fulltext" target="_blank" >https://journals.biologists.com/jcs/article-abstract/doi/10.1242/jcs.258228/268958/Divergent-estimates-of-ratio-between-Na-Ca2?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.258228" target="_blank" >10.1242/jcs.258228</a>

Result language

angličtina

Original language name

Divergent estimates of the ratio between Na+-Ca2+ current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes

Original language description

The ratio between Na+-Ca2+ exchange current densities in t-tubular and surface membranes of rat ventricular cardiomyocytes (JNaCa-ratio) estimated from electrophysiological data published to date yields strikingly different values between 1.7 and nearly 40. Possible reasons for such divergence were analysed by Monte-Carlo simulations assuming both normal and log-normal distribution of the measured data. The confidence intervals CI95 of the mean JNaCa-ratios computed from the reported data showed an overlap of values between 1 and 3 and between 0.3 and 4.3 in the case of normal and log-normal distribution, respectively. Further analyses revealed that the published high values likely result from a large scatter of data due to transmural differences in JNaCa, dispersion of cell membrane capacitances and variability in incomplete detubulation. Taking into account the asymmetric distribution of measured data, the reduction of mean current densities after detubulation, and the substantially smaller CI95 of lower values of the mean JNaCa-ratio, the values between 1.6 and 3.2 may be considered as the most accurate estimates. This implies that 40 to 60% of Na+-Ca2+ exchanger is located at the t-tubular membrane of adult rat ventricular cardiomyocytes.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30500 - Other medical sciences

Project

<a href="/en/project/NV16-30571A" target="_blank" >NV16-30571A: Clinical significance and electrophysiological evaluation of KCNQ1 gene mutation c.926C>T (p.T309I) as a possible long QT syndrome founder mutation</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

1477-9137

Volume of the periodical

134

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

jcs258228

UT code for WoS article

000681395800011

EID of the result in the Scopus database

2-s2.0-85112478836