Clustered DNA damage on subcellular level: effect of scavengers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F14%3A00436873" target="_blank" >RIV/61389005:_____/14:00436873 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00411-014-0557-2" target="_blank" >http://dx.doi.org/10.1007/s00411-014-0557-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00411-014-0557-2" target="_blank" >10.1007/s00411-014-0557-2</a>

Result language

angličtina

Original language name

Clustered DNA damage on subcellular level: effect of scavengers

Original language description

Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusterswas found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connec

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/LD12008" target="_blank" >LD12008: Effect of high LET radiation on specific interaction of proteins with DNA.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Radiation and Environmental Biophysics

ISSN

0301-634X

e-ISSN

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Volume of the periodical

53

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

705-712

UT code for WoS article

000344177800008

EID of the result in the Scopus database

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