Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F08%3A00310795" target="_blank" >RIV/61389013:_____/08:00310795 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions
Original language description
Copolymers of 2-(2-hydroxyethoxy)ethyl methacrylate and ethylene dimethacrylate in the form of homogeneous membrane sheets were modified by chelating groups of iminodiacetic acid (IDA) in a two step reaction. The obtained sorbents showed high chelating capacity for Ni(II), Cu(II) and Fe(III) ions (up to 1.3 mmol/g). The potential use of immobilized Ni(II)-IDA complexes for sorption of murine immunoglobulin G by the immobilized metal affinity (IMA) method was tested. The sorption behaviour was characterized by a Langmuir-Freundlich adsorption isotherm. It was shown that the chelating membranes could be used for the sorption of low amounts of protein.
Czech name
Membrány z chelatujících polymerů. Příprava a použití pro sorpce kovů a imunoglobulinu G na imobilizovaných iontech Ni(II)
Czech description
Kopolymery 2-(2-hydroxyethoxy)ethyl methakrylátu a ethylen dimethakrylátu ve tvaru homogenní membrány byly modifikovány chelatačními skupinami kyseliny iminodioctové (IDA) dvoustupňovou reakcí. Získaný sorbent má vysokou sorpční kapacitu pro kovové iontyNi(II), Cu(II) a Fe(III) (max. 1.3 mmol/g). Ověřeno bylo použití imobilizovaného komplexu Ni(II)-IDA pro sorpci myšího imunoglobulinu G chromatografickou metodou IMAC. Sorpční vlastnosti jsou charakterizovány Langmuir-Freundlichovou adsorpční isotermou.Membrány jsou použitelné pro sorpci malých množství proteinů.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F02%2F1327" target="_blank" >GA203/02/1327: Hybrid supports from Polyhedral Oligomeric Silsesquiozane and chitin derivatives for membrane applications</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Polymer Bulletin
ISSN
0170-0839
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
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UT code for WoS article
000257727300002
EID of the result in the Scopus database
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