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Effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F09%3A00324002" target="_blank" >RIV/61389013:_____/09:00324002 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378041:_____/09:00324002 RIV/00023001:_____/09:00001965 RIV/00216208:11130/09:5416

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling

  • Original language description

    Maghemite nanoparticles with various coatings were prepared by the coprecipitation method and characterized by transmission electron microscopy, dynamic light scattering and IR in terms of morphology, size, polydispersity and surface coating. The labeling efficiency and the viability of both rat and human mesenchymal stem cells labeled with Endorem (R), poly(L-lysine) (PLL)-modified Endorem (R), uncoated gamma-Fe2O3, D-mannose-, PLL- or poly(N,N-dimethylacrylamide) (PDMAAm)-coated gamma-Fe2O3 nanoparticles were compared. Coated gamma-Fe2O3 nanoparticles labeled cells better than did Endorem (R). High relaxation rates and in vitro magnetic resonance imaging of cells labeled with coated nanoparticles showed clearly visible contrast compared with unlabeled cells or cells labeled with Endorem (R).

  • Czech name

    Vliv různých povlaků magnetických nanočástic na účinnost značení kmenových buněk

  • Czech description

    Maghemitové nanočástice s různými povlaky byly připraveny koprecipitací a charakterizovány transmisní elektronovou mikroskopií, dynamickým rozptylem světla a IČ spektroskopií co do morfologie, velikosti, polydisperzity a povrchového povlaku. Byla porovnána účinnost značení a viabilita jak potkaních, tak i lidských mesenchymových kmenových buněk značených Endoremem, Endoremem modifikovaným poly(L-lysinem) (PLL), nepovlečenými ?-Fe2O3 nanočásticemi a ?-Fe2O3 nanočásticemi povlečenými D-manózou, PLL nebo poly(N,N-dimethylakrylamidem) (PDMAAm). Povlečené ?-Fe2O3 nanočástice značily buňky lépe než Endorem.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FR - Pharmacology and apothecary chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Magnetism and Magnetic Materials

  • ISSN

    0304-8853

  • e-ISSN

  • Volume of the periodical

    321

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

    000265278000048

  • EID of the result in the Scopus database