Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00445996" target="_blank" >RIV/61389013:_____/15:00445996 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/C5RA09280A" target="_blank" >http://dx.doi.org/10.1039/C5RA09280A</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/C5RA09280A" target="_blank" >10.1039/C5RA09280A</a>

Result language
angličtina
Original language name
Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus
Original language description
Systemic lupus erythematosus (SLE) is a heterogeneous, inflammatory and multisystem autoimmune disease in which antinuclear antibodies are present in blood often years before clinical symptoms occur. Isolating the antibodies is thus of crucial importanceto confirm the diagnosis and prognosis of patients with some autoimmune diseases. Isolation can be performed advantageously using magnetic microspheres, which offer easy and quick manipulation with a magnet and avoid sample dilution. Here, we developedcalf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) (POEMA?His) microspheres using a multiple-stage swelling technique followed by His immobilization. Magnetic POEMA?His microspheres were characterized using scanning and transmission electron microscopy, SQUID, ATR FT-IR spectroscopy, elemental analysis and atomic absorption spectrometry. The microspheres were successfully used for rapid purification of the anti-histone immunoglobulins (IgGs) from blood serum samples of
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Project
<a href="/en/project/7E12053" target="_blank" >7E12053: Nanosystems for early Diagnosis of Neurodegenerative Diseases</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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