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Differential scanning fluorimetry illuminates silk feedstock stability and processability

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00452866" target="_blank" >RIV/61389013:_____/16:00452866 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1039/C5SM02036k" target="_blank" >http://dx.doi.org/10.1039/C5SM02036k</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1039/C5SM02036k" target="_blank" >10.1039/C5SM02036k</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Differential scanning fluorimetry illuminates silk feedstock stability and processability

  • Original language description

    The ability to design and implement silk feedstock formulations for tailored spinning has so far eluded the bioengineers. Recently, the high throughput screening technique of differential scanning fluorimetry (DSF) demonstrated the link between the instability transition temperature (Ti) and the processability of the silk feedstock. Using DSF we screened a large set of chemicals known to affect solvent quality. A multivariate analysis of the results shows that, regardless of the diversity of chemicals,three groupings are significantly distinguishable: G1 = similar to native silk; G2 = largely dominated by electrostatic interactions; and G3 = dominated by chelating interactions. We propose a thermodynamic analysis based on a pre- and post-transition fit to estimate the van't Hoff enthalpies (?Hv) and the instability temperature (Ti). Our analysis shows that the ?Ti and ?Hv values were distinct: G1 (?Ti = 0.23 0.2; ?Hv = 159.1 5.6 kcal mol1), G2 (?Ti = 7.3 0.7; ?Hv = 191.4 5.5 kcal mol1

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/EE2.3.30.0029" target="_blank" >EE2.3.30.0029: BIOPOL: BIOpolymer POstdoc Laboratory and educative center</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Soft Matter

  • ISSN

    1744-683X

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    8

  • Pages from-to

    255-262

  • UT code for WoS article

    000366863800030

  • EID of the result in the Scopus database

    2-s2.0-84951130318