Curcumin-bortezomib loaded polymeric nanoparticles for synergistic cancer therapy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00475193" target="_blank" >RIV/61389013:_____/17:00475193 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/17:10361465
Result on the web
<a href="http://dx.doi.org/10.1016/j.eurpolymj.2017.05.036" target="_blank" >http://dx.doi.org/10.1016/j.eurpolymj.2017.05.036</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.eurpolymj.2017.05.036" target="_blank" >10.1016/j.eurpolymj.2017.05.036</a>
Alternative languages
Result language
angličtina
Original language name
Curcumin-bortezomib loaded polymeric nanoparticles for synergistic cancer therapy
Original language description
A series of well-defined methoxy-poly(ethylene glycol)-block-polylactic acid (mPEG-b-PLA) diblock copolymers were successfully synthesized, characterized and used for the construction of anticancer nanoparticle delivery system. Nanoparticles (NPs) based on these polymers were prepared by employing the nanoprecipitation method, and they were non-covalently loaded with curcumin, curcumin-bortezomib model or curcumin-bortezomib complex (curc-BTZ). Both curcumin and bortezomib are rather hydrophobic and poorly water-soluble potent anticancer drugs with synergic effects forming together a pH-sensitive complex, stable at pH of blood plasma, yet hydrolytically labile at mildly acidic milieu typical for endosomes and interstitial space in solid tumors. PEG-Curcumin-loaded and curc-BTZ-loaded NPs with 100–150 nm size showed the maximum cellular uptake by HeLa, MCF-7 and MDA-MB 231 cells after 3 h. The NPs were located in the cytoplasm of the cells but not inside the nucleus. Bare NPs did not induce any cytotoxicity in the same cell lines in in vitro experiments, even at very high concentrations (up to 800 µg/mL). NPs containing curcumin were cytotoxic with an IC50 of 25 µg/mL, which corresponds to 2.5 µg/mL of loaded curcumin. These results show that the efficacy of curcumin is significantly enhanced when using the NPs as carriers. The efficiency was further augmented through the complexation of BTZ with curcumin. When using free curc-BTZ-complex, MCF-7 cells were more sensitive to the free complex 18.8 nM (IC50) than MDA-MB-231 cells 122.4 nM (IC50). Nanoparticle formulations with these drugs caused significant cytotoxicity with 7.5 nM (IC50) and 59.2 nM (IC50) after 24 h of the treatment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Polymer Journal
ISSN
0014-3057
e-ISSN
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Volume of the periodical
93
Issue of the periodical within the volume
August
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
116-131
UT code for WoS article
000407186200012
EID of the result in the Scopus database
2-s2.0-85019995345