Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00511603" target="_blank" >RIV/61389013:_____/20:00511603 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/20:00511603
Result on the web
<a href="https://www.confer.cz/nanocon/2019" target="_blank" >https://www.confer.cz/nanocon/2019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2019.8445" target="_blank" >10.37904/nanocon.2019.8445</a>
Alternative languages
Result language
angličtina
Original language name
Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
Original language description
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent immunoassay labels due to their ability to emit light of shorter wavelengths under near-infrared excitation (anti-Stokes emission), which prevents autofluorescence, minimizes light scattering, and thus reduces the optical background interference. These unique photoluminescent properties allow counting of individual biomolecules labeled with UCNPs by conventional wide-field epiluminescence microscopy and enable the development of single-molecule (digital) immunoassays. We have introduced a novel label based on UCNPs conjugated with streptavidin via poly(ethylene glycol) and applied it in a digital upconversion-linked immunosorbent assay (ULISA) for the detection of a cancer biomarker prostate specific antigen (PSA). The digital readout based on counting of individual immunocomplexes improved the sensitivity 16× compared to conventional analog readout and allowed to reach a limit of detection (LOD) of 23 fg·mL−1 (800 aM). Human serum samples were successfully analyzed achieving an excellent correlation with electrochemiluminescence reference method. The conjugates of UCNPs with streptavidin are also suitable for the detection of pathogenic bacterium Melissococcus plutonius, the causative agent of honeybee disease European foulbrood. The ULISA assay provided an LOD of 340 CFU·mL−1 and successfully analyzed real samples of bees, larvae and bottom hive debris. Due to the high reliability and relatively simple detection scheme, the digital ULISA can pave the way for a new generation of digital immunoassays with a strong potential for commercialization.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/LTAB19011" target="_blank" >LTAB19011: Sensitive bioanalytical tools for the surveillance of honey bee diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
NANOCON Conference Proceedings - International Conference on Nanomaterials
ISBN
978-808729495-6
ISSN
2694-930X
e-ISSN
—
Number of pages
6
Pages from-to
311-316
Publisher name
Tanger Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Oct 16, 2019
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000664115400051