The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00532733" target="_blank" >RIV/61389013:_____/20:00532733 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/20:00532733 RIV/00216208:11130/20:10410852 RIV/00023001:_____/20:00079182

Result on the web

<a href="https://link.springer.com/article/10.1007/s11064-019-02790-9" target="_blank" >https://link.springer.com/article/10.1007/s11064-019-02790-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11064-019-02790-9" target="_blank" >10.1007/s11064-019-02790-9</a>

Result language

angličtina

Original language name

The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages

Original language description

Superparamagnetic iron oxide nanoparticles (SPIOn) are widely used as a contrast agent for cell labeling. Macrophages are the first line of defense of organisms in contact with nanoparticles after their administration. In this study we investigated the effect of silica-coated nanoparticles (gamma-Fe2O3-SiO2) with or without modification by an ascorbic acid (gamma-Fe2O3-SiO2-ASA), which is meant to act as an antioxidative agent on rat peritoneal macrophages. Both types of nanoparticles were phagocytosed by macrophages in large amounts as confirmed by transmission electron microscopy and Prusian blue staining, however they did not substantially affect the viability of exposed cells in monitored intervals. We further explored cytotoxic effects related to oxidative stress, which is frequently documented in cells exposed to nanoparticles. Our analysis of double strand breaks (DSBs) marker gamma H2AX showed an increased number of DSBs in cells treated with nanoparticles. Nanoparticle exposure further revealed only slight changes in the expression of genes involved in oxidative stress response. Lipid peroxidation, another marker of oxidative stress, was not significantly affirmed after nanoparticle exposure. Our data indicate that the effect of both types of nanoparticles on cell viability, or biomolecules such as DNA or lipids, was similar, however the presence of ascorbic acid, either bound to the nanoparticles or added to the cultivation medium, worsened the negative effect of nanoparticles in various tests performed. The attachment of ascorbic acid on the surface of nanoparticles did not have a protective effect against induced cytotoxicity, as expected.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neurochemical Research

ISSN

0364-3190

e-ISSN

—

Volume of the periodical

45

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

159-170

UT code for WoS article

000511696200014

EID of the result in the Scopus database

2-s2.0-85064253798