Versatile bioconjugation strategies of PEG-modified upconversion nanoparticles for bioanalytical applications

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00534143" target="_blank" >RIV/61389013:_____/20:00534143 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/20:00116881
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.biomac.0c00459" target="_blank" >10.1021/acs.biomac.0c00459</a>

Result language
angličtina
Original language name
Versatile bioconjugation strategies of PEG-modified upconversion nanoparticles for bioanalytical applications
Original language description
Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis. They are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction. Alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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