Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00552077" target="_blank" >RIV/61389013:_____/22:00552077 - isvavai.cz</a>
Alternative codes found
RIV/00023736:_____/22:00013332
Result on the web
<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262484" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262484</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0262484" target="_blank" >10.1371/journal.pone.0262484</a>
Alternative languages
Result language
angličtina
Original language name
Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome
Original language description
Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological and pathophysiological functions are poorly understood in hematological malignancies.Here, we investigated proteome changes in the exosome-rich fraction of the plasma of myelodysplastic syndrome patients and healthy donors. Exosome-rich fraction of the plasma was isolated using ExoQuick™: proteomes were compared and statistically processed, proteins were identified by nanoLC-MS/MS and verified using the ExoCarta and QuickGO databases. Mann-Whitney and Spearman analyses were used to statistically analyze the data. 2D western blot was used to monitor clusterin proteoforms. Statistical analyses of the data highlighted clusterin alterations as the most significant. 2D western blot showed that the clusterin changes were caused by posttranslational modifications. Moreover, there was a notable increase in the clusterin proteoform in the exosome-rich fraction of plasma of patients with more severe myelodysplastic syndrome, this corresponded with a simultaneous decrease in their plasma. This specific clusterin proteoform seems to be a promising biomarker for myelodysplastic syndrome progression.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GA20-10845S" target="_blank" >GA20-10845S: Blood plasma individual variability and pathophysiology and their influence on the interactions with synthetic antifouling surfaces</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
1932-6203
Volume of the periodical
17
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
e0262484
UT code for WoS article
000741060700010
EID of the result in the Scopus database
2-s2.0-85122617681