Cyclic strain mitigates nanoparticle internalization by vascular smooth muscle cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00554892" target="_blank" >RIV/61389013:_____/22:00554892 - isvavai.cz</a>

Result on the web

<a href="https://www.dovepress.com/cyclic-strain-mitigates-nanoparticle-internalization-by-vascular-smoot-peer-reviewed-fulltext-article-IJN" target="_blank" >https://www.dovepress.com/cyclic-strain-mitigates-nanoparticle-internalization-by-vascular-smoot-peer-reviewed-fulltext-article-IJN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S337942" target="_blank" >10.2147/IJN.S337942</a>

Result language

angličtina

Original language name

Cyclic strain mitigates nanoparticle internalization by vascular smooth muscle cells

Original language description

Intravascular delivery of nanoparticles for theranostic application permits direct interaction of nanoparticles and vascular cells. Since vascular smooth muscle cells (VSMCs), the major components of the vascular wall, are constantly subjected to mechanical stimulation from hemodynamic influence, we asked whether cyclic strain may modulate internalization of magnetic nanoparticles (MNPs) by cultured VSMCs. Cyclic strain (1 Hz and 10%) was applied with Flexcell system in cultured VSMCs from rats, with cell-associated MNPs (MNPcell) determined by a colorimetric iron assay. Transmission and scanning electron microscopy were used for morphology studies. Confocal microscopy was used to demonstrate distribution of actin assembly in VSMCs. Incubation of poly(acrylic acid) (PAA)-coated MNPs with VSMCs for 4 h induced microvilli formation and MNP internalization. Application of cyclic strain for 4– 12 h significantly reduced MNPcell by up to 65% (p < 0.05), which was associated with blunted microvilli and reduced vesicle size/cell, but not vesicle numbers/cell. Confocal microscopy demonstrated that both cyclic strain and fibronectin coating of the culture plate reduced internalized MNPs, which were co-localized with vinculin. Furthermore, cytochalasin D reduced MNPcell, suggesting a role of actin polymerization in MNP uptake by VSMCs, however, a myosin II ATPase inhibitor, blebbistatin, exhibited no effect. Cyclic strain also attenuated uptake of PAA-MNPs by LN-229 cells and uptake of poly-L-lysine-coated MNPs by VSMCs. In such a dynamic milieu, cyclic strain may impede cellular internalization of nanocarriers, which spares the nanocarriers and augments their delivery to the target site in the lumen of vessels or outside of the circulatory system.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

<a href="/en/project/GC20-02177J" target="_blank" >GC20-02177J: Antioxidant phenolic compound-modified magnetic nanoparticles for treatment of oxidative stress-related pathologies: Study of nano-biointerfaces</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

1178-2013

Volume of the periodical

17

Issue of the periodical within the volume

5 March

Country of publishing house

NZ - NEW ZEALAND

Number of pages

13

Pages from-to

969-981

UT code for WoS article

000766607300003

EID of the result in the Scopus database

2-s2.0-85126412691