Polymer-antimicrobial peptide constructs with tailored drug-release behavior
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00567633" target="_blank" >RIV/61389013:_____/23:00567633 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22310/23:43926856 RIV/60461373:22330/23:43926856
Result on the web
<a href="https://www.mdpi.com/1999-4923/15/2/406" target="_blank" >https://www.mdpi.com/1999-4923/15/2/406</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/pharmaceutics15020406" target="_blank" >10.3390/pharmaceutics15020406</a>
Alternative languages
Result language
angličtina
Original language name
Polymer-antimicrobial peptide constructs with tailored drug-release behavior
Original language description
Microbial resistance is one of the main problems of modern medicine. Recently, antimicrobial peptides have been recognized as a novel approach to overcome the microbial resistance issue, nevertheless, their low stability, toxicity, and potential immunogenic response in biological systems have limited their clinical application. Herein, we present the design, synthesis, and preliminary biological evaluation of polymer-antibacterial peptide constructs. The antimicrobial GKWMKLLKKILK-NH2 oligopeptide (PEP) derived from halictine, honey bee venom, was bound to a polymer carrier via various biodegradable spacers employing the pH-sensitive or enzymatically-driven release and reactivation of the PEP’s antimicrobial activity. The antibacterial properties of the polymer-PEP constructs were assessed by a determination of the minimum inhibitory concentrations, followed by fluorescence and transmission electron microscopy. The PEP exerted antibacterial activity against both, gram-positive and negative bacteria, via disruption of the bacterial cell wall mechanism. Importantly, PEP partly retained its antibacterial efficacy against Staphylococcus epidermidis, Escherichia coli, and Acinetobacter baumanii even though it was bound to the polymer carrier. Indeed, to observe antibacterial activity similar to the free PEP, the peptide has to be released from the polymer carrier in response to a pH decrease. Enzymatically-driven release and reactivation of the PEP antimicrobial activity were recognized as less effective when compared to the pH-sensitive release of PEP.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/GA20-04790S" target="_blank" >GA20-04790S: Polymer terapeutics based on antimicrobial peptides for multi-resistence bakteria species treatment</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Pharmaceutics
ISSN
1999-4923
e-ISSN
1999-4923
Volume of the periodical
15
Issue of the periodical within the volume
2
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
406
UT code for WoS article
000940898800001
EID of the result in the Scopus database
2-s2.0-85149117262