PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00568881" target="_blank" >RIV/61389013:_____/23:00568881 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/23:00568881 RIV/46747885:24510/23:00010962

Result on the web

<a href="https://www.dovepress.com/plclpcl-dressings-with-platelet-lysate-and-growth-factors-embedded-in--peer-reviewed-fulltext-article-IJN" target="_blank" >https://www.dovepress.com/plclpcl-dressings-with-platelet-lysate-and-growth-factors-embedded-in--peer-reviewed-fulltext-article-IJN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S393890" target="_blank" >10.2147/IJN.S393890</a>

Result language

angličtina

Original language name

PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration

Original language description

The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ϵ-caprolactone) and poly(ϵ-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

1178-2013

Volume of the periodical

18

Issue of the periodical within the volume

3 February

Country of publishing house

NZ - NEW ZEALAND

Number of pages

16

Pages from-to

595-610

UT code for WoS article

000928064400001

EID of the result in the Scopus database

2-s2.0-85147762343