Cryopreservation of apheresis platelets treated with riboflavin and UV light
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00570648" target="_blank" >RIV/61389013:_____/23:00570648 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/24:00558342 RIV/61383082:_____/23:00001274 RIV/68407700:21460/23:00373168 RIV/00216208:11110/23:10465966 and 2 more
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1473050222002798?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1473050222002798?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.transci.2022.103580" target="_blank" >10.1016/j.transci.2022.103580</a>
Alternative languages
Result language
angličtina
Original language name
Cryopreservation of apheresis platelets treated with riboflavin and UV light
Original language description
Pathogen reduction technology (PRT) is increasingly used in the preparation of platelets for therapeutic transfusion. As the Czech Republic considers PRT, we asked what effects PRT may have on the recovery and function of platelets after cryopreservation (CP), which we use in both military and civilian blood settings. 16 Group O apheresis platelets units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing, 15 similarly collected units were frozen without PRT as controls. All units were processed with 5–6% DMSO, frozen at − 80 °C, stored > 14 days, and reconstituted in thawed AB plasma. After reconstitution, all units were assessed for: platelet count, mean platelet volume (MPV), platelet recovery, thromboelastography, thrombin generation time, endogenous thrombin potential (ETP), glucose, lactate, pH, pO2, pCO2, HCO3, CD41, CD42b, CD62, Annexin V, CCL5, CD62P, and aggregates > 2 mm and selected units for Kunicki score. PRT treated platelet units had lower platelet number (247 vs 278 ×109/U), reduced thromboelastographic MA (38 vs 62 mm) and demonstrated aggregates compared to untreated platelets. Plasma coagulation functions were largely unchanged. Samples from PRT units showed reduced platelet number, reduced function greater than the reduced number would cause, and aggregates. While the platelet numbers are sufficient to meet the European standard, marked platelets activation with weak clot strength suggest reduced effectiveness.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Transfusion and Apheresis Science
ISSN
1473-0502
e-ISSN
1878-1683
Volume of the periodical
62
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
103580
UT code for WoS article
000996453300001
EID of the result in the Scopus database
2-s2.0-85138766172