Synthesis and structure optimization of star copolymers as tunable macromolecular carriers for minimal immunogen vaccine delivery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F24%3A00597653" target="_blank" >RIV/61389013:_____/24:00597653 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/24:00597653

Result on the web

<a href="https://pubs.acs.org/doi/10.1021/acs.bioconjchem.4c00273" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.bioconjchem.4c00273</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.bioconjchem.4c00273" target="_blank" >10.1021/acs.bioconjchem.4c00273</a>

Result language

angličtina

Original language name

Synthesis and structure optimization of star copolymers as tunable macromolecular carriers for minimal immunogen vaccine delivery

Original language description

Minimal immunogen vaccines are being developed to focus antibody responses against otherwise challenging targets, including human immunodeficiency virus (HIV), but multimerization of the minimal peptide immunogen on a carrier platform is required for activity. Star copolymers comprising multiple hydrophilic polymer chains (“arms”) radiating from a central dendrimer unit (“core”) were recently reported to be an effective platform for arraying minimal immunogens for inducing antibody responses in mice and primates. However, the impact of different parameters of the star copolymer (e.g., minimal immunogen density and hydrodynamic size) on antibody responses and the optimal synthetic route for controlling those parameters remains to be fully explored. We synthesized a library of star copolymers composed of poly[N-(2-hydroxypropyl)methacrylamide] hydrophilic arms extending from poly(amidoamine) dendrimer cores with the aim of identifying the optimal composition for use as minimal immunogen vaccines. Our results show that the length of the polymer arms has a crucial impact on the star copolymer hydrodynamic size and is precisely tunable over a range of 20–50 nm diameter, while the dendrimer generation affects the maximum number of arms (and therefore minimal immunogens) that can be attached to the surface of the dendrimer. In addition, high-resolution images of selected star copolymer taken by a custom-modified environmental scanning electron microscope enabled the acquisition of high-resolution images, providing new insights into the star copolymer structure. Finally, in vivo studies assessing a star copolymer vaccine comprising an HIV minimal immunogen showed the criticality of polymer arm length in promoting antibody responses and highlighting the importance of composition tunability to yield the desired biological effect.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioconjugate Chemistry

ISSN

1043-1802

e-ISSN

1520-4812

Volume of the periodical

35

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

1218-1232

UT code for WoS article

001282046200001

EID of the result in the Scopus database

2-s2.0-85199943997