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Liraglutide-conjugated poly(methyl vinyl ether-alt-maleic acid)-coated core-shell upconversion nanoparticles for theranostics of diabetes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00637800" target="_blank" >RIV/61389013:_____/25:00637800 - isvavai.cz</a>

  • Alternative codes found

    RIV/67985823:_____/25:00637800 RIV/00023001:_____/25:00085775 RIV/46747885:24530/25:00014635 RIV/00216208:11110/25:10500135

  • Result on the web

    <a href="https://pubs.acs.org/doi/10.1021/acsami.5c11275" target="_blank" >https://pubs.acs.org/doi/10.1021/acsami.5c11275</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/acsami.5c11275" target="_blank" >10.1021/acsami.5c11275</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Liraglutide-conjugated poly(methyl vinyl ether-alt-maleic acid)-coated core-shell upconversion nanoparticles for theranostics of diabetes

  • Original language description

    In the diagnostics of diabetes, specific targeting of drugs (e.g., liraglutide) to insulin-deficient β-cells with their simultaneous noninvasive imaging is currently needed. In this report, liraglutide (LGL)-conjugated poly(methyl vinyl ether-alt-maleic acid) (PMVEMA)-coated core–shell NaYF4:Yb,Er,Fe@NaYF4:Nd upconversion nanoparticles (CS-UCNPs) have been developed, thoroughly physicochemically characterized, and evaluated in vivo. Novel codoping of Fe2+, Yb3+, and Er3+ ions in the host NaYF4 induced upconversion emission in the red region at both 980 and 808 nm excitation, making the particles suitable for deep-tissue imaging. Surface functionalization with PMVEMA provided colloidal stability and facilitated covalent conjugation with LGL, enabling targeted binding to GLP-1 receptors on pancreatic β-cells, increasing glucose-stimulated insulin secretion from isolated Langerhans islets. Biocompatibility of CS-UCNP@PMVEMA-LGL nanoparticles was confirmed by the trypan blue dye exclusion assay. When the fluorescent dye Flamma was conjugated to the nanoparticles, in vivo fluorescence imaging revealed significantly enhanced accumulation of CS-UCNP@PMVEMA-LGL-Flamma nanoparticles in the pancreas 24 h after intramuscular injection compared with intravenous administration, with luminescence intensity approximately doubled. The improved pancreatic targeting efficiency was attributed to enhanced binding to GLP-1 receptors. Confocal microscopy and elemental analysis confirmed receptor-mediated uptake of the nanoparticles by internalization and their localization within pancreatic β-cells. These findings highlight the potential of CS-UCNP@PMVEMA-LGL nanoparticles as biocompatible targetable imaging agents with future applications in pancreatic diagnostics.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10404 - Polymer science

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    ACS Applied Materials and Interfaces

  • ISSN

    1944-8244

  • e-ISSN

    1944-8252

  • Volume of the periodical

    17

  • Issue of the periodical within the volume

    30

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    42863-42876

  • UT code for WoS article

    001530081300001

  • EID of the result in the Scopus database

    2-s2.0-105012784149