Delivery of Human iPSC-Derived RPE Cells in Healthy Minipig Retina Results in Interaction Between Photoreceptors and Transplanted Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00639550" target="_blank" >RIV/61389013:_____/25:00639550 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/25:00639550 RIV/67985904:_____/25:00639550 RIV/00216208:11120/25:43928336 RIV/00216208:11310/25:10497019 RIV/00064173:_____/25:43928336
Result on the web
<a href="https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/advs.202412301?getft_integrator=clarivate&src=getftr&utm_source=clarivate" target="_blank" >https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/advs.202412301?getft_integrator=clarivate&src=getftr&utm_source=clarivate</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/advs.202412301" target="_blank" >10.1002/advs.202412301</a>
Alternative languages
Result language
angličtina
Original language name
Delivery of Human iPSC-Derived RPE Cells in Healthy Minipig Retina Results in Interaction Between Photoreceptors and Transplanted Cells
Original language description
In late stages of inherited and acquired retinal diseases such as Stargardt disease (STGD) or dry age-related macular degeneration (AMD), loss of retinal pigment epithelia (RPE) cells and subsequently photoreceptors in the macular area result in a dramatic decline of central visual function. Repopulating this area with functional RPE cells may prevent or decline the progression of photoreceptor loss. In the present study, the viability, survival, and integration of human induced pluripotent stem cell (hiPSC)-derived RPE cells (hiPSC-RPE) is assessed generated using clinical-grade protocol and cultured on a clinically relevant scaffold (poly-L-lactide-co-D, L-lactide, PDLLA) after subretinal implantation in immunosuppressed minipigs for up to 6 weeks. It is shown that transplanted hiPSC-RPE cells maintain the RPE cell features such as cell polarity, hexagonal shape, and cell-cell contacts, and interact closely with photoreceptor outer segments without signs of gliosis or neuroinflammation throughout the entire period of examination. In addition, an efficient immunosuppressing strategy with a continuous supply of tacrolimus is applied. Continuous verification and improvement of existing protocols are crucial for its translation to the clinic. The results support the use of hiPSC-RPE on PDLLA scaffold as a cell replacement therapeutic approach for RPE degenerative diseases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Advanced Science
ISSN
2198-3844
e-ISSN
2198-3844
Volume of the periodical
12
Issue of the periodical within the volume
20
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
2412301
UT code for WoS article
001458150100001
EID of the result in the Scopus database
2-s2.0-105002069298