Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by lnositol 3,4,5,6-Tetrakisphosphate.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F02%3A56023023" target="_blank" >RIV/61389030:_____/02:56023023 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by lnositol 3,4,5,6-Tetrakisphosphate.

Original language description

Oscillatory growth of pollen tubes has been correlated with oscillatory influxes of the cations Ca2+, H+, and K+. Using an ion-specific vibrating probe, a new circuit was identified that involves oscillatory efflux of the anion Cl - at the apex and steady influx along the tube starting at 12 (j.m distal to the tip. This spatial coupling of influx and efflux sites predicts that a vectorial flux of Cl~ ion traverses the apical region. The Cl~ channel blockers 4,4'-diisothiocyanatostilbene-2,2'-disulfonicacid (DIDS) and 5-nitro-2-(3-phenylpropylamino)benzoic acid completely inhibited tobacco pollen tube growth at 80 and 20 (xM, respectively. Cl~ channel blockers also induced increases in apical cell volume. The apical 50 (Jim of untreated pollen tubes had a mean cell volume of 3905 75 |xm3. DIDS at 80 |j.M caused a rapid and lethal cell volume increase to 6206 171 (j.m3, which is at the point of cell bursting at the apex. DIDS was further demonstrated to disrupt Cl~ efflux from the apex,

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

<a href="/en/project/LN00A081" target="_blank" >LN00A081: Signaling pathways in plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell

ISSN

1040-4651

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

N/A

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

2233-2249

UT code for WoS article

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EID of the result in the Scopus database

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