Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033010" target="_blank" >RIV/61389030:_____/03:56033010 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of molecular cytogenetics and physical mapping of ribosomal RNA genes in Lupinus.

Original language description

Identification of individual chromosomes in Lupinus is not possible due to gradient in size and in similar morphology. To overcome this problem, molecular cytogenetics was developed for Lupinus. As an initial step in karyotype analysis, fluorescent in situ hybridization (FISH) was performed to determine genomic distribution of rRNA genes in L. hispanicus, L. luteus and L. x hispanicoluteus.It was found that all three diploid species posses two chromosome pairs carrying 18S-5.8S-25S rDNA and one chromosome pair carrying 5S rDNA. The use of probes for DNA permitted unambiguous identification of three different pairs of chromosomes and revealed conservation of the number of rDNA loci among the three species. The study represents the first step in physicalmapping of Lupinus genome through FISH by providing distinct chromosome lendmarks.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA521%2F03%2F0595" target="_blank" >GA521/03/0595: Targeted isolation of molecular markers and comparative genome mapping in chickpea, field bean and garden pea</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

N/A

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

211-215

UT code for WoS article

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EID of the result in the Scopus database

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