Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033075" target="_blank" >RIV/61389030:_____/03:56033075 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots.

Original language description

2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to theinhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescin

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/LN00A081" target="_blank" >LN00A081: Signaling pathways in plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0032-0919

e-ISSN

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Volume of the periodical

164

Issue of the periodical within the volume

5

Country of publishing house

FR - FRANCE

Number of pages

10

Pages from-to

823-832

UT code for WoS article

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EID of the result in the Scopus database

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