Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033106" target="_blank" >RIV/61389030:_____/03:56033106 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion.

Original language description

Regulation of cytokinin oxidase (CKX) activity in relation to enzyme glycosylation and secretion was studied in wild-type (WT) and transgenic conditionally isopentenyltransferase gene (ipt)-expressing (IPT) tobacco (Nicotiana tabacum L. cv. Wisconsin 38)cell suspensions, calli and leaves. An increase in endogenous cytokinin content due to the tetracycline (Tc)-induced derepression of the ipt gene transcription or surface application of N6-benzylaminopurine (BA) resulted in significant enhancement of CKX activity in all these plant materials. As revealed by Concanavalin A-Sepharose 4B chromatography the cytokinin-induced enhancement of CKX activity was associated predominantly with the N-glycoform of the enzyme (10- to 15-fold increase) in calli and leaves. Application of BA to the culture media of WT and IPT cell suspensions and the derepression of the ipt by Tc substantially enhanced endogenous levels of isoprenoid cytokinins and CKX activity in both cells and the culture medium. Mos

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

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Volume of the periodical

117

Issue of the periodical within the volume

1

Country of publishing house

DK - DENMARK

Number of pages

11

Pages from-to

11-21

UT code for WoS article

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EID of the result in the Scopus database

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