Molecular cytogenetics and cytometry of banana (Musa spp.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00107482" target="_blank" >RIV/61389030:_____/04:00107482 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/04:00002310

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular cytogenetics and cytometry of banana (Musa spp.)

Original language description

This work focused on the development of flow cytometry and molecular cytogenetics for Musa, and on the use of new tools for detailed and more efficient characterisation and analysis of its genome at the nuclear and chromosomal levels. Using flow cytometry, genome size of Musa was determined for the first time, and the extent of its variation was assessed. The method was invaluable for the rapid estimation of ploidy levels and detection of mixoploidy. While the method remains an important research tool,it is being increasingly used in Musa breeding programmes. Molecular cytogenetical methods that permit physical mapping of DNA sequences to Musa chromosomes were developed and used first to obtain physical chromosome landmarks and to characterise variousrepetitive DNA sequences. A novel method for preparation of high molecular weight DNA has been developed, and work is under way to construct the first Musa B-genome BAG library. The results obtained represent major steps forward in ou...

Czech name

Molekulární cytogenetika a cytometrie banánovníku (Musa spp.)

Czech description

Práce je zaměřena na vývoj průtokové cytometrie a molekulární cytogenetiky u banánovníku. Pomocí průtokové cytometrie byla poprvé stanovena velikost genomu banánovníku a její variabilita. Molekulárně cytogenetické metody umožňující fyzické mapování DNA sekvencí na chromozómech byly použity pro získaní markerů pro některé chromozómy banánovníku a pro charakterizaci repetitvních dNA sekvencí. Získané výsledky představují důležitý krok k pochopení struktury a evoluce genomu banánovníku.

Type

C - Chapter in a specialist book

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/IAA6038204" target="_blank" >IAA6038204: Construction of banana bacterial artificial chromosome (BAC) library for physical genome mapping</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Banana Improvement: Cellular, Molecular Biology, and Induced Mutations

ISBN

1-57808-340-0

Number of pages of the result

15

Pages from-to

229-244

Number of pages of the book

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Publisher name

Science Publishers, Inc

Place of publication

Enfield

UT code for WoS chapter

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