Flow cytometric analysis of variation in the level of nuclear DNA endoreduplication in the cotyledons amongst Vigna radiata cultivars

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00110101" target="_blank" >RIV/61389030:_____/04:00110101 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Flow cytometric analysis of variation in the level of nuclear DNA endoreduplication in the cotyledons amongst Vigna radiata cultivars

Original language description

Most of the differentiated plant cells replicate their nuclear DNA but there is no chromosome condensation, strand separation, nor cytokinesis, resulting in multiple copies of the genome, and the phenomenon is called endoreduplication. In this communication we report the distribution pattern of DNA endoreduplication in the nuclei of the mature cotyledons of six cultivars of Vigna radiata (mungbean). It has shown that the maximum ploidy level present in the cotyledonary tissues of V radiata is 64C, where2C is DNA amount of unreplicated diploid nucleus, except in the cultivar ML 337, where only up to 32C DNA content was noted. Considerable difference in the level of C-value distribution amongst different cultivars of V radiata has been found in the present investigation, This is the first report on flow cytometric analysis showing variation in the level of nuclear endoreduplication amongst the cultivars of a grain legume.

Czech name

Analýza variability DNA endoreduplikace v děložních lístcích kultivarů Vigna radiata

Czech description

V tomto článku je popsáno rozdělení DNA endoreduplikace v jádrech děložních lístků šesti kultivarů Vigna radiata. Nejvyšši nalezená ploidie byla 64C. Byly nalezeny statisticky průkazné rozdíly v rozsahu endoreduplikace u studovaných kultivarů. Jedná se oprvní zprávu týkající se analýzy variability rozsahu endoreduplikace u luštěnin pomocí průtokové cytometrie.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/IBS5038104" target="_blank" >IBS5038104: Molecular cytogenetic and cytometric methods for breeding of grasses and trifolium</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Caryologia

ISSN

0008-7114

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

3

Country of publishing house

IT - ITALY

Number of pages

5

Pages from-to

262-266

UT code for WoS article

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EID of the result in the Scopus database

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