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Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00028411" target="_blank" >RIV/61389030:_____/05:00028411 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants

  • Original language description

    Effects of the infection with tobacco mosaic virus (TMV) and potato virus Y (PVY) on chloroplasts from susceptible tobacco plants were determined. Changes in ribonucleases (RNases), phosphomonoesterase (PME), phosphodiesterase (PDE), glucose-6-phosphatedehydrogenase (G6P DH), 6-phosphogluconate dehydrogenase (6PG DH), glucokinase (GK), and fructokinase (FK) activities in thylakoid/envelope and stroma fractions were studied. Slight increase in the activities of PME, PDE, G6P DH and 6PG DH of thylakoid/envelope fraction as well as of RNases, PME, PDE, G6P DH, 6PG DH, GK and FK of stroma fraction was found in chloroplasts isolated from leaf tissues infected with PVY. Infection with TMV produced higher increase in enzymes activities in chloroplasts; especially, PME, G6P DH and 6PG DH in fraction of thylakoid/envelope, and RNases, PME, PDE, G6P DH, 6PG DH, and GK in stroma fraction.

  • Czech name

    Změny regulačních enzymů vedoucích k biosyntéze virové RNA v chloroplastech z PVY a TMV infikovaných rostlin tabáku

  • Czech description

    Účinky infekce Y-virem bramboru (PVY) a virem mozaiky tabáku (TMV) byly studovány v chloroplastech susceptibilních rostlin tabáku. Změny v aktivitách enzymů [ribonukleázy (RNases), fosfomonoesterázy (PME), fosfodiesterázy (PDE), glukóza-6-fosfát dehydrogenázy (G6P DH), 6-fosfoglukonát dehydrogenázy (6PG DH), glukokinázy (GK) a fruktokinázy(FK)] byly měřeny jak ve stromální frakci tak v thylakoidní frakci obsahující i obalovou membránu chloroplastu. Slabé zvýšení aktivit PME, PDE, G6P DH a 6PG DH bylo nalezeno v thylakoidní frakci stejně jako RNases, PME, PDE, G6P DH, 6PG DH, GK a FK ve stromální frakci z chloroplastů isolovaných z listů infikovaných PVY. TMV infekce způsobila mnohem nárůst enzymové activity v chloroplastech, obzvlášť PME, G6P DH a 6PGDH v thylakoidní frakci, a RNases, PME, PDE, G6P DH, 6PG DH, a GK ve stromální frakci.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA522%2F02%2F0708" target="_blank" >GA522/02/0708: The role of chloroplasts in mechanisms involved in replication and translation of viral-RNA in connection with host resistance</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biologia Plantarum

  • ISSN

    0006-3134

  • e-ISSN

  • Volume of the periodical

    49

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    4

  • Pages from-to

    471-474

  • UT code for WoS article

  • EID of the result in the Scopus database